Handbook of Media for Clinical and Public Health Microbiology

The detection and/or isolation and identification of pathogenic microorganisms is critical for the laboratory diagnosis of infectious diseases. With growth-dependant methods providing reliable means for identifying pathogens, traditional culturing continues to play an integral role in the detection and characterization of known and "new" microbial pathogens. Microbiologists, therefore, rely on a variety of media for the detection, isolation, characterization, and identification of primary and opportunistic microbial pathogens. The Handbook of Media for Clinical and Public Health Microbiology provides a compilation of the formulations, methods of preparation, and applications for media used in clinical and public health microbiology laboratories. It is a significant update to the Handbook of Media for Clinical Microbiology, expanding the coverage to media used for public health epidemiological investigations of disease outbreaks and including media used for the detection of pathogens in foods and environmental samples. Comprising both classic and modern media, the handbook describes almost 1,800 types of media, listed alphabetically, including new media for the cultivation of emerging bacteria, fungi, and viruses that are causing major medical problems around the world. Examples of emerging pathogens are extended-spectrum beta-lactamase (ESBL)-producing bacteria, Escherichia coli O157:H7, methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant enterococci (VRE), and carbapenem-resistant Enterobacteriaceae (CRE). Many of the new media contain chromogenic or fluorogenic substrates that permit rapid detection of specific pathogens. The handbook’s format allows easy reference to information needed to prepare media for cultivating clinically relevant microorganisms. It also contains descriptions of expected results for organisms that are important for the examination of foods, water, and other specimens of public health significance as well as clinical specimens. Features Provides an authoritative, easy-to-use handbook of media for the culture of pathogenic microorganisms in the clinical and public health laboratories Organizes media into sections making it easier to use for those performing bacteriological analyses Describes commercial sources and storage/shelf life of media Describes media for emerging pathogens that cause diseases with high mortality rates Includes newly developed media designed for rapid detection of microbes, which contain chromogenic or fluorogenic substrates Table of Contents Diagnostic Microbiology: Isolation and Identification of Pathogens Isolation and Culture Procedures Media for the Isolation and Identification of Microorganisms of Public Health Concern Media for the Isolation and Identification of Microorganisms from Clinical and Public Health Specimens References Web Resources Alphabetical Listings of Bacteriological, Mycological, Protozoan, and Viral Culture Media. Ronald M. Atlas, Ph.D., is a professor of biology at the University of Louisville, Kentucky. He is a Fellow of the American Academy of Microbiology. He has authored several microbiology textbooks and handbooks of microbiological media. His research has included development of diagnostic systems for pathogenic microorganisms. He has served on the editorial boards of Applied and Environmental Microbiology, Bio- Science, Biotechniques, Environmental Microbiology, Biosecurity, and Bioterrorism, and Journal of Industrial Microbiology. He is the previous editor of Critical Reviews in Microbiology. He also has served as president of the American Society for Microbiology. James W. Snyder, Ph.D., is a professor in the Department of Pathology and Laboratory Medicine at the University of Louisville, School of Medicine, Kentucky, and serves as the director of microbiology for the University of Louisville Hospital. Dr. Snyder is a diplomate of the American Board of Medical Microbiology (ABMM) and a Fellow in the American Academy of Microbiology. He is a member of the Critical Reviews in Microbiology editorial board and serves on the Professional Affairs Laboratory Practices Committee of the American Society for Microbiology. He serves as a reviewer for the Journal of Clinical Microbiology and member and coordinating editor of the Laboratory Response Network Sentinel Level Laboratory Protocols for Biothreat and Emerging Infectious Disease Agents. He maintains an active research program in both applied and basic clinical microbiology, including molecular techniques for the detection of pathogens and tracking of microbial incidence and in vitro activity of antibiotics in support of local and national surveillance programs.