This fully updated guide examines techniques in equine theriogenology
The second edition of Equine Reproductive Procedures provides equine practitioners, veterinary students, and equine theriogenologists with a fully updated, practical guide to techniques in equine reproductive practice. This easy-to-use resource covers skills-based information with a clinical focus, taking an easy-to-follow step-by-step approach.
The book covers topics related to the reproductive management of horses, including diagnostic techniques and therapeutic procedures for stallions, mares, and foals. Procedures highlighted in the book are supported with clear descriptions and photographs. Readers will also find a list of required supplies for the procedure and a reference list.
· Provides step-by-step descriptions of techniques related to equine theriogenology
· Serves as a valuable practice tool
· Offers 39 new techniques not found in the first edition
· Includes key updates to existing techniques
Those studying equine reproduction and specialists in theriogenology will find this an essential ‘how-to’ guide for their practice library.
Table of contents
1 Reproductive Evaluation of the Mare
Patrick M. McCue
2 Teasing
Patrick M. McCue
3 Tail Wrap and Preparation/Washing of the Perineum
John J. Dascanio
4 Placement of a Tail Rope
John J. Dascanio
5 Perineal Conformation Evaluation
John J. Dascanio
6 Removal of a Persistent Hymen
John J. Dascanio
7 Palpation of the Reproductive Tract of the Non-Pregnant Mare
Patrick M. McCue
8 Ultrasound Evaluation of the Non-Pregnant Mare
Patrick M. McCue
9 Prediction of Ovulation
Patrick M. McCue
10 Speculum Examination of the Vagina
John J. Dascanio
11 Digital Examination of the Vagina/Cervix
Sofie Sitters
12 Uterine Culture Collection: Swab/Brush
John J. Dascanio
13 Antimicrobiotic Sensitivity Testing
Jillian Bishop and Patrick M. McCue
14 Microbiology: Microbial Culture
Patrick M. McCue and Jillian Bishop
15 Microbiology: Gram Stain
Jillian Bishop and Patrick M. McCue
16 qPCR Assay for the Diagnosis of Infectious Endometritis
Ryan A. Ferris and Patrick M. McCue
17 Uterine Cytology Collection: Swab/Brush
John J. Dascanio and Ryan A. Ferris
18 Uterine Culture/Cytology: Low Volume Lavage
John J. Dascanio
19 Endometrial Biopsy
Patrick M. McCue
20 Hysteroscopic Examination of the Uterus
Patrick M. McCue
21 Endometrial Cyst Removal
John J. Dascanio
22 Chromosomal Analysis
Terje Raudsepp
23 Endocrinological Examination of the Mare
Patrick M. McCue
24 Laparoscopic Examination of the Uterus and Ovaries
Dean A. Hendrickson
25 Evaluation of the Mammary Gland
Patrick M. McCue
26 Antisperm Antibody Testing
Patrick M. McCue
27 Starch Granule Test for the Evaluation of Oviductal Patency
Sofie Sitters and John J. Dascanio
28 Fluorescent Microspheres Test for Evaluation of Oviductal Patency
Sofie Sitters and John J. Dascanio
29 Oviductal Flush Procedure for the Evaluation of Oviductal Patency
Sofie Sitters and Patrick M. McCue
30 Prostaglandin E2 Treatment for Blocked Oviducts
Patrick M. McCue, Sofie Sitters and Charles F. Scoggin
31 Prostaglandin E1 Treatment for Blocked Oviducts
Patrick M. McCue and Charles F. Scoggin
32 Hysterocoscopic Hydrotubation of the Oviducts
Charles F. Scoggin
33 Transvaginal Ovarian Biopsy
Claire E. Card
34 Uterine Lavage
John J. Dascanio
35 Uterine Infusion
John J. Dascanio
36 Therapeutics for Infectious Endometritis
Ryan A. Ferris
37 Bacterial Biofilm and Endometritis
Ryan A. Ferris
38 Latent Uterine Bacterial Infections.
Morten Rønn Petersen and Anders Miki Bojesen
39 Acupuncture in Mare Reproduction
Kristina Lu and Lauren Javernick
40 Restraint for Breeding
John J. Dascanio
41 Natural Service: Pasture Breeding
Patrick M. McCue
42 Natural Service: In-hand Breeding
Charles F. Scoggin
43 Breeding Stitches
Charles F. Scoggin
44 Reinforcement Breeding
Charles F. Scoggin
45 Breeding with Fresh or Cooled Semen
John J. Dascanio and Patrick M. McCue
46 Insemination Through a Vaginal Speculum
Patrick M. McCue
47 Breeding with Frozen Semen
John J. Dascanio
48 Deep Horn Insemination
John J. Dascanio
49 Hysteroscopic (Low Dose) Insemination
Patrick M. McCue
50 Transvaginal oocyte pickup (OPU)
Jennifer N. Hatzel
51 Packaging and Transport of Oocytes
Jennifer N. Hatzel
52 Intracytoplasmic Sperm Injection (ICSI)
Jennifer N. Hatzel and JoAnne Stokes
53 Caslick Operation or Vulvoplasty
John J. Dascanio
54 Contagious Equine Metritis Testing
John J. Dascanio
55 Management of Seasonal Anestrus: Artificial Photoperiod
Patrick M. McCue
56 Management of Seasonal Anestrus: Hormone Therapy
Patrick M. McCue
57 Hormone Therapy in Cycling Mares
Patrick M. McCue
58 Estrus Suppression
Patrick M. McCue
59 Intra-uterine Devices for Estrus Suppression
Carlos M. Gradil
60 Immunocontraception
Patrick M. McCue
61 Harvesting and Shipping Ovaries for Oocyte Recovery
Patrick M. McCue
62 Embryo Collection
Patrick M. McCue
63 Embryo Evaluation
Patrick M. McCue
64 Embryo Biopsy
Patrick M. McCue
65 Embryo Packaging for Cooled Transport
Patrick M. McCue
66 Embryo Cryopreservation
Patrick M. McCue
67 Non-Surgical Embryo Transfer
Patrick M. McCue
68 Autogenous Transfer of ICSI Derived Embryos
Jennifer N. Hatzel
69 Palpation of the Pregnant Mare Per Rectum
Sofie Sitters
70 Ultrasound Examination of the Pregnant Mare
Patrick M. McCue
71 Speculum Examination of the Pregnant Mare
Sofie Sitters
72 Determination of Equine Fetal Sex 55 – 200 days
Richard D. Holder
73 Assessment of Late Term Fetal Well-being
John J. Dascanio
74 Combined Thickness of the Uterus and Placenta
Patrick M. McCue
75 Placentitis – Diagnosis and Treatment
Justin W. McNaughten and Margo L. Macpherson
76 Doppler Ultrasonography
John J. Dascanio
77 Prediction of Fetal Age
John J. Dascanio
78 Twin Reduction: Manual Technique
John J. Dascanio
79 Twin Reduction: Transvaginal Aspiration
John J. Dascanio
80 Twin Reduction: Cranio-Cervical Dislocation
Karen Wolfsdorf
81 Twin Reduction: Thoracic Compression
Patrick M. McCue
82 Twin Reduction: Transabdominal Fetal Cardiac Puncture
John J. Dascanio
83 Elective Termination of Pregnancy
John J. Dascanio
84 Abortion Diagnostic Evaluation: Sample Collection in the Field and Submission
Julie T. Cecere and John J. Dascanio
85 Endocrine Evaluation of Pregnancy
Patrick M. McCue
86 Treatment of Vaginal Varicosities
John J. Dascanio
87 Manual Correction of Uterine Torsion
John J. Dascanio
88 Preparation of the Mare for Foaling
John J. Dascanio
89 Assessment of Mammary Gland Secretions to Predict Foaling
John J. Dascanio
90 Assessment of pH of Mammary Gland Secretions to Predict Foaling
Patrick M. McCue
91 Monitoring of Peri-Parturient Mares Using Video and Web Cameras
Ryan A. Ferris
92 Foaling Alert: Vulvar Device
John J. Dascanio
93 Positional Labor Alert Devices
Ryan A. Ferris
94 Induction of Parturition
John J. Dascanio
95 Normal Foaling
John J. Dascanio
96 Premature Separation of the Placenta
Patrick M. McCue
97 Dystocia Management
Patrick M. McCue and Sofie Sitters
98 Dystocia Correction
John J. Dascanio
99 Fetotomy
John J. Dascanio
100 Epidural
John J. Dascanio
101 Induction of Lactation to Create a Nurse Mare
Pouya Dini and Peter F. Daels
102 Screening the Pregnant Mare to Prevent Neonatal Isoerythrolysis
Patrick M. McCue
103 Evaluation of Colostrum Specific Gravity
Patrick M. McCue
104 Evaluation of Colostrum Quality: Brix Refractometry
Patrick M. McCue
105 Colostrum Banking
Patrick M. McCue
106 Obtaining Milk from the Mare
John J. Dascanio
107 Placental Evaluation
Patrick M. McCue
108 Removal of Retained Placenta
John J. Dascanio
109 Abdominocentesis in the Postpartum Mare
John J. Dascanio
110 Uterine Prolapse Treatment
John J. Dascanio
111 Buhner Needle Placement of a Perivulvar Suture
John J. Dascanio
112 Breeding Soundness Evaluation
Patrick M. McCue
113 Training of a Stallion to Use a Phantom
John J. Dascanio
114 Missouri Artificial Vagina
John J. Dascanio and Lynda M.J. Miller
115 Colorado Model Artificial Vagina
Patrick M. McCue
116 Roanoke Artificial Vagina
John J. Dascanio
117 Hannover Artificial Vagina
Sofie Sitters
118 Teaser Stallions
Charles F. Scoggin
119 Standing Semen Collection
John J. Dascanio
120 Collection of Semen using an Open-Ended Artificial Vagina
Patrick M. McCue
121 Chemical Ejaculation
Patrick M. McCue
122 Preparation of a Jump Mare or a Mare for Natural Cover
John J. Dascanio
123 Washing the Penis
John J. Dascanio
124 Evaluation of Sexual Behavior in the Stallion
John J. Dascanio Sue M. McDonnell
125 Enhancement of Sexual Interest and Response in Stallions
Sue M. McDonnell
126 Management of Ejaculation Difficulty in Stallions
Sue M. McDonnell
127 Calibrated Spectrophotometer Evaluation of Sperm Concentration
John J. Dascanio
128 Hemocytometer Evaluation of Sperm Concentration
John J. Dascanio
129 NucleoCounter® Evaluation of Sperm Concentration and Viability
Patrick M. McCue
130 Visual Evaluation of Sperm Motility
Patrick M. McCue
131 Computer-Assisted Sperm Analysis
John J. Dascanio and Lynda M. J. Miller
132 Eosin-Nigrosin Staining in the Evaluation of Sperm
Julie T. Cecere
133 Evaluation of Sperm Morphology
Leonardo Brito
134 Determination of Daily Sperm Output
Patrick M. McCue
135 Measurement of Testicular Size and Estimation of Daily Sperm Output
Patrick M. McCue
136 Wet Mount Evaluation of Sperm
John J. Dascanio
137 Diff-Quik Evaluation of Round Cells and Sperm
John J. Dascanio and Lynda M. J. Miller
138 Electron Microscopy of Constituents of Seminal Ejaculates
D. N. Rao Veeramachaneni, BVSc, MScVet, PhD
139 Bacterial Culture in the Stallion
John J. Dascanio
140 Evaluation of pH and Osmolarity of Semen
Patrick M. McCue
141 Alkaline Phosphatase: A Marker for Ejaculation
Patrick M. McCue
142 Diagnosis and Management of Urospermia
John J. Dascanio
143 Hemospermia
Patrick M. McCue
144 Semen Extenders and Sperm Media
Patrick M. McCue
145 Preparation of Semen for Cooled Transport
Etta A. Bradecamp
146 Packing Semen for Cooled Transport
Etta A. Bradecamp
147 Longevity Testing of Sperm
John J. Dascanio
148 Calculation of g Force for Centrifuging Semen
John J. Dascanio
149 Centrifugation of Semen: Standard Technique
Etta A. Bradecamp
150 Centrifugation of Semen: Cushion Technique
Etta A. Bradecamp
151 Centrifugation of Semen: Selection of Motile Sperm Using a Single Layer Colloid Technique
Etta A. Bradecamp
152 Use of a Sperm Filter
Marco A. Alvarenga, Lorenzo G. T. M. Segabinazzi
153 Semen Freezing
Patrick M. McCue
154 Refreezing Stallion Semen
Patrick M. McCue
155 Thawing Frozen Semen
John J. Dascanio
156 Semen Processing for ICSI
Patrick M. McCue
157 Preparing a Vapor Shipper
Julie T. Cecere and Paul Loomis
158 Loading a Vapor Shipper
Julie T. Cecere and Paul Loomis
159 Maintaining a Long-Term Frozen Semen Storage Tank
John J. Dascanio
160 International Transport of Frozen Semen
Paul Loomis
161 Harvesting and Shipping Testes
John J. Dascanio
162 Epididymal Sperm Recovery
John J. Dascanio and Lynda M.J. Miller
163 Endoscopic Examination of the Urethra
Patrick M. McCue
164 Endoscopic Guided Cannulation of the Seminal Vesicles
Patrick M. McCue
165 Palpation and Ultrasonography of the Testis, Epididymis, and Spermatic Cord
Sofie Sitters
166 Palpation and Ultrasonography of the Accessory Sex Glands
Sofie Sitters
167 Radiographic Examination of the Penis
Patrick M. McCue
168 Testicular Biopsy and Aspiration
John J. Dascanio
169 Assessment of Sperm Plasma Membrane Integrity and Viability: Propidium Iodide/SYBR-14
Amanda I. Glazar and Patrick M. McCue
170 Sperm Chromatin Structure Assay
Charles Love
171 Hypo-Osmotic Swelling Test
Patrick M. McCue
172 Assessment of Sperm Acrosomal Status: FITC PNA
Amanda I. Glazar and Patrick M. McCue
173 Assessment of Sperm Mitochondrial Function: JC-1 and Rhodamine 123
Amanda I. Glazar and Patrick M. McCue
174 Equine Viral Arteritis Testing
G. Reed Holyoak and Udeni B. R. Balasuriya
175 Diagnostic Endocrinology: Baseline Hormone Levels
Patrick M. McCue
176 Diagnostic Endocrinology: GnRH Stimulation Tests
Patrick M. McCue
177 Diagnostic Endocrinology: hCG Stimulation Test
Patrick M. McCue
178 Diagnostic Endocrinology: Estrogen Conjugate Assay
Patrick M. McCue
179 Diagnostic Tests for Cryptorchidism
Patrick M. McCue
180 Probang Apparatus
Julie T. Cecere
181 Support Apparatus for Paraphimosis
John J. Dascanio
182 Equine Castration Techniques
Stacy Anderson
183 Stallion Acupuncture
Rhonda A Rathgeber
184 Birth Resuscitation
Patrick M. McCue
185 Foal Handling and Restraint
Undine Christmann
186 Madigan Foal Squeeze Technique
John Madigan
187 Evaluation of Passive Transfer
Patrick M. McCue
188 Colostral Administration via Naso-Gastric Intubation
John J. Dascanio
189 Sedation of the foal
Undine Christmann
190 Intravenous Catheter Placement in the Foal
Undine Christmann
191 Plasma Therapy in the Foal
Undine Christmann
192 Acupuncture in the Neonate
Peter Morresey
193 Routine Care
Patrick M. McCue
194 Foal Rejection
Patrick M. McCue
195 Fostering a Foal onto a Nurse Mare
Peter F Daels and Pouya Dini
196 Entropion
John J. Dascanio
197 Umbilical Ultrasound in the Foal
Undine Christmann
198 Management of Soft Tissue Laxity in Foals
Stacy Anderson
199 Management of Angular Limb Deformities
Stacy Anderson
200 Management of Congenital Flexural Limb Deformities
Stacy Anderson
